Barnet have lost their Football League status after a 2-0 defeat at Sixfields against Play-Off dwellers Northampton Town.
The Bees started the day outside of the League 2 drop zone but a win for AFC Wimbledon saw them replace the Wombles in the bottom two.
Roy O'Donovan and Luke Guttridge scored second-half goals for the Cobblers to sink Edgar Davids' men, while Gary Alexander and Jack Midson found the net in AFC Wimbledon's 2-1 victory against Fleetwood Town.
Barnet have been in The Football League since promotion from the Conference in 2005 and avoided relegation on the final day last season, finishing third from bottom, but they were unable to avoid the drop back into non-league this time around.
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